PROCEED AMP3 User Manual download pdf

Infinium

HD Assay Gemini, Manual Protocol

Experienced User Card

Catalog # WG-901-3002 Page 1 of 28

Part # 11311015 Rev. A

Day 2 Day 3

Make AMP3

Hands-on:

~20 min/16 samples

Incubation: 20–24 hours

Reagents

0.1N NaOH

MP1

AMM

Output

AMP3 Plate with

Amplified DNA

XStain HD

BeadChip

Hands-on:~3hours

Dry: 60 min

Reagents

RA1

95% Formamide /

1mMEDTA

PB1

XC1

XC2

XC3

XC4

TEM

STM

ATM

Output

BeadChip

Precip AMP3

Hands-on:~30min/plate

Incubation:50min

Dry: 60 min

Reagents

2-propanol

PA1

Output

AMP3 Plate

Fragment AMP3

Hands-on:~30min

Incubation:60min

Reagents

FRG

Output

AMP3 Plate

Resuspend AMP3

Hands-on:~30min

Incubation:60min

Reagents

RA1

Output

AMP3 Plate

Hyb Duo BeadChip

Hands-on:

~30 min/8 BeadChips

Incubation: 16–24 hours

Reagents

PB2

Output

BeadChip

Wash BeadChip

Hands-on:

~20 min/8 BeadChips

Reagents

PB1

Output

BeadChip

Image BeadChip

iScan System Scan Time:

35 min/BeadChip

BeadArray Reader Scan

Time: 45 min/BeadChip

Output

mage and Data Files

Day 1

Quantitate DNA

Hands-on: 30 min/plate

Fluorometer: 5 min/plate

Reagents

Lambda DNA

PicoGreen dsDNA

1X TE

Output

Sample QDNA Plate with

Quantitated DNA

Pre-Amp

Optional

InfiniumLIMS

Post-Amp

Cold Storage

Option

Overnight

Incubation

Fill in the lab tracking

form and the sample

sheet as you perform

the assay

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Experienced User Card 1

Summary of Contents

Page 1 - Experienced User Card

Infinium® HD Assay Gemini, Manual ProtocolExperienced User CardCatalog # WG-901-3002 Page 1 of 28Part # 11311015 Rev. ADay 2 Day 3Make AMP3Hands-on:~2

Page 2

Infinium® HD Assay Gemini, Manual Protocol (Post-AMP)Experienced User CardCatalog # WG-901-3002 Page 10 of 28Part # 11311015 Rev. A[ ] 15. Place the

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Infinium® HD Assay Gemini, Manual Protocol (Post-AMP)Experienced User CardCatalog # WG-901-3002 Page 11 of 28Part # 11311015 Rev. AEstimated TimeHands

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Infinium® HD Assay Gemini, Manual Protocol (Post-AMP)Experienced User CardCatalog # WG-901-3002 Page 13 of 28Part # 11311015 Rev. AEstimated TimeHands

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Infinium® HD Assay Gemini, Manual Protocol (Post-AMP)Experienced User CardCatalog # WG-901-3002 Page 14 of 28Part # 11311015 Rev. A[ ] 4. Combine th

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Infinium® HD Assay Gemini, Manual Protocol (Post-AMP)Experienced User CardCatalog # WG-901-3002 Page 15 of 28Part # 11311015 Rev. A[ ] 4. Visually i

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Infinium® HD Assay Gemini, Manual Protocol (Post-AMP)Experienced User CardCatalog # WG-901-3002 Page 16 of 28Part # 11311015 Rev. A[ ] 5. If you are

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Infinium® HD Assay Gemini, Manual Protocol (Post-AMP)Experienced User CardCatalog # WG-901-3002 Page 17 of 28Part # 11311015 Rev. AEstimated TimeHands

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Infinium® HD Assay Gemini, Manual Protocol (Post-AMP)Experienced User CardCatalog # WG-901-3002 Page 18 of 28Part # 11311015 Rev. A[ ] 5. Remove the

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Infinium® HD Assay Gemini, Manual Protocol (Post-AMP)Experienced User CardCatalog # WG-901-3002 Page 19 of 28Part # 11311015 Rev. AAssemble Flow-Throu

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Infinium® HD Assay Gemini, Manual Protocol (Post-AMP)Experienced User CardCatalog # WG-901-3002 Page 21 of 28Part # 11311015 Rev. AEstimated TimeHands

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Infinium® HD Assay Gemini, Manual Protocol (Post-AMP)Experienced User CardCatalog # WG-901-3002 Page 22 of 28Part # 11311015 Rev. APreparation [ ] G

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Infinium® HD Assay Gemini, Manual Protocol (Post-AMP)Experienced User CardCatalog # WG-901-3002 Page 23 of 28Part # 11311015 Rev. A[ ] c. 250 μl ATM

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Infinium® HD Assay Gemini, Manual Protocol (Post-AMP)Experienced User CardCatalog # WG-901-3002 Page 24 of 28Part # 11311015 Rev. ADo not let it sit f

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Infinium® HD Assay Gemini, Manual Protocol (Post-AMP)Experienced User CardCatalog # WG-901-3002 Page 25 of 28Part # 11311015 Rev. AEstimated TimeScann

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Infinium® HD Assay Gemini, Manual Protocol (Post-AMP)Experienced User CardCatalog # WG-901-3002 Page 27 of 28Part # 11311015 Rev. AEstimated Time1–2 h

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Infinium® HD Assay Gemini, Manual Protocol (Post-AMP)Experienced User CardCatalog # WG-901-3002 Page 28 of 28Part # 11311015 Rev. AWhen the BeadArray

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Infinium® HD Assay Gemini, Manual Protocol (Pre-AMP)Experienced User CardCatalog # WG-901-3002 Page 3 of 28Part # 11311015 Rev. AEstimated TimeHands-o

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Infinium® HD Assay Gemini, Manual Protocol (Pre-AMP)Experienced User CardCatalog # WG-901-3002 Page 4 of 28Part # 11311015 Rev. ADilute PicoGreen[ ]

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Infinium® HD Assay Gemini, Manual Protocol (Pre-AMP)Experienced User CardCatalog # WG-901-3002 Page 5 of 28Part # 11311015 Rev. AEstimated TimeHands-o

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Infinium® HD Assay Gemini, Manual Protocol (Pre-AMP)Experienced User CardCatalog # WG-901-3002 Page 6 of 28Part # 11311015 Rev. A[ ] 8. Dispense 150

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Infinium® HD Assay Gemini, Manual Protocol (Post-AMP)Experienced User CardCatalog # WG-901-3002 Page 7 of 28Part # 11311015 Rev. AEstimated TimeHands-

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Infinium® HD Assay Gemini, Manual Protocol (Post-AMP)Experienced User CardCatalog # WG-901-3002 Page 8 of 28Part # 11311015 Rev. A• Proceed to Precip

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Infinium® HD Assay Gemini, Manual Protocol (Post-AMP)Experienced User CardCatalog # WG-901-3002 Page 9 of 28Part # 11311015 Rev. AEstimated TimeHands-