PROCEED AMP3 User Manual download pdf (Page 18)

Infinium

HD Assay Gemini, Manual Protocol (Post-AMP)

Experienced User Card

Catalog # WG-901-3002 Page 18 of 28

Part # 11311015 Rev. A

[ ] 5. Remove the IntelliHyb seal from each BeadChip as follows:

[ ] a. Using powder-free gloved hands, hold the BeadChip in one

hand with your thumb and forefinger on the long edges of the

BeadChip. The BeadChip may also be held with the thumb and

forefinger on the short edges of the BeadChip. In either case

avoid contact with the sample inlets. The barcode should be

facing up and be closest to you, and the top side of the

BeadChip should be angled slightly away from you.

[ ] b. Remove the entire seal in a single, rapid motion by pulling it off

in a diagonal direction. Start with a corner on the barcode end

and pull with a continuous upward motion away from you and

towards the opposite corner on the top side of the BeadChip.

Do not stop and start the pulling action. Do not touch the

exposed active areas.

[ ] 6. Immediately and carefully slide each BeadChip into the wash rack one at

a time, making sure that the BeadChip is completely submerged in the

PB1.

[ ] 7. Repeat steps 5 and 6 until all BeadChips are transferred to the

submerged wash rack. The wash rack holds up to 8 BeadChips.

[ ] 8. Once all BeadChips are in the wash rack, move the wash rack up and

down for 1

minute, breaking the surface of the PB1 with gentle, slow

agitation.

[ ] 9. Move the wash rack to the other wash dish containing PB1. Make sure

the BeadChips are completely sub merged.

[ ] 10. Move the wash rack up and down for 1 minute, breaking the surface of

the PB1 with gentle, slow agitation.

[ ] 11. If you are processing more than 8 BeadChips:

[ ] a. Complete the steps in the next section, Assemble Flow-Through

Chambers, for the first eight BeadChips.

[ ] b. Place the assembled Flow-Through Chambers of the first eight

BeadChips on the lab bench in a horizontal position.

[ ] c. Repeat steps 3 through 11 from this section for any additional

BeadChips. Use new PB1 for each set of eight BeadChips.

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PROCEED AMP3 User Manual Page 18