PROCEED AMP3 User Manual download pdf (Page 24)

Infinium

HD Assay Gemini, Manual Protocol (Post-AMP)

Experienced User Card

Catalog # WG-901-3002 Page 24 of 28

Part # 11311015 Rev. A

Do not let it sit for more than 10 minutes.

[ ] 7. Move the BeadChip staining rack into the XC4 dish. The barcodes should

face away from you and the locking arms towards you.

[ ] 8. Slowly move the staining rack up and down 10 times, breaking the

surface of the reagent.

[ ] 9. Soak for 5 minutes.

[ ] 10. Lift the staining rack out of the solution and place it horizontally on a

tube rack, with the BeadChip barcodes facing

up.

[ ] 11. Remove the BeadChips from the staining rack with locking tweezers,

working from top to bottom. Place each BeadChip on a tube rack to dry.

Remove the staining rack handle before removing the BeadChips below

it.

[ ] 12. Dry the BeadChips in the vacuum desiccator for 50–55 minutes at

508

mm Hg (0.68 bar). Each desiccator can hold 8 BeadChips.

[ ] 13. Clean the underside of the BeadChip with a ProStat EtOH wipe.

[ ] 14. Clean and store the glass back plates and Hyb Chamber components.

[ ] 15. Do one of the following:

• Proceed to Image BeadChip on iScan System or Image BeadChip on

BeadArray Reader.

• Store the BeadChips in the Illumina BeadChip Slide Storage Box

inside a vacuum desiccator at room temperature. Image the

BeadChips within 72 hours.

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PROCEED AMP3 User Manual Page 24