PROCEED AMP3 User Manual download pdf (Page 23)

Infinium

HD Assay Gemini, Manual Protocol (Post-AMP)

Experienced User Card

Catalog # WG-901-3002 Page 23 of 28

Part # 11311015 Rev. A

[ ] c. 250 μl ATM and incubate for 10 minutes.

[ ] d. 450 μl XC3 and incubate for 1 minute. Repeat once, and then

wait 5 minutes.

[ ] e. 250 μl STM and incubate for 10 minutes.

[ ] f. 450 μl XC3 and incubate for 1 minute. Repeat once, and then

wait 5 minutes.

[ ] g. 250 μl ATM and incubate for 10 minutes.

[ ] h. 450 μl XC3 and incubate for 1 minute. Repeat once, and then

wait 5 minutes.

[ ] i. 250 μl STM and incubate for 10 minutes.

[ ] j. 450 μl XC3 and incubate for 1 minute. Repeat once, and then

wait 5 minutes.

[ ] 3. Immediately remove the Flow-Through Chambers from the Chamber

Rack and place horizontally on a lab bench at room temperature.

Wash and Coat

[ ] 1. Dispense PB1 into a wash dish as follows, and then cover the dish:

• For 8 Beadchips, dispense 310 ml

• For 16 Beadchips, dispense 300 ml

• For 24 BeadChips, dispense 285 ml

[ ] 2. Place the staining rack inside the wash dish. The locking arms and tab

should face

towards you.

[ ] 3. For each BeadChip:

• Use the dismantling tool to remove the two metal clamps from the

Flow-Through Chamber.

• Remove the glass back plate, the spacer, and then the BeadChip.

• Immediately place each BeadChip into the staining rack that is in the

PB1 wash dish, with the barcode facing

away from you. Place half of

the BeadChips above the handle and half below. All chips should b e

completely submerged.

[ ] 4. Slowly move the staining rack up and down 10 times, breaking the

surface of the reagent.

[ ] 5. Soak for 5 minutes.

[ ] 6. Dispense XC4 into a wash dish as follows:

• For 8 Beadchips, dispense 310 ml

• For 16 Beadchips, dispense 300 ml

• For 24 BeadChips, dispense 285 ml

CAUTION

Handle the BeadChips only by the edges or the barcode

end. Do not let the BeadChips dry out.

CAUTION

Do not leave the BeadChips in the PB1 for more than

30 minutes.

[ ]

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PROCEED AMP3 User Manual Page 23

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