Infinium
®
HD Assay Gemini, Manual Protocol (Post-AMP)
Experienced User Card
Catalog # WG-901-3002 Page 27 of 28
Part # 11311015 Rev. A
Estimated Time
1–2 hours warmup for the BeadArray Reader (first use of the day only)
45 minutes to scan each BeadChip using BeadScan 3.2 FastScan settings
Preparation
[ ] If this is the first time the BeadArray Reader is being used today, follow
the steps described in this section.
[ ] On the lab tracking form, record the following for each BeadChip:
• Scanner ID
• Scan date
Initializing the
BeadArray
Reader (Daily)
If this is the first time the scanner is being used today, follow these steps.
[ ] 1. Locate the power switch on the lower-left side of the BeadArray Reader
back panel and turn it to the ON position.
[ ] 2. Wait for the ready indicator to stop flashing.
[ ] 3. Open the BeadScan software.
[ ] 4. Log in and click Scan.
Imaging
BeadChip
When the BeadArray Reader is initialized, follow these steps to perform the
scanning process.
[ ] 1. From the Docking Fixture listbox, select BeadChip.
[ ] 2. Check the Data Repository path and the Decode Map path in the
Settings area.
[ ] 3. Copy the decode map (*.dmap) files for each BeadChip from the
BeadChip CD to the Decode Map path directory.
[ ] 4. For each BeadChip:
[ ] a. Place the BeadChip into the BeadArray Reader tray.
[ ] b. If either the Sentrix Type or Scan Settings are not correct, click
Browse (...) to open the Select Scan Settings dialog b ox.
[ ] c. Select the appropriate scan method and click Select.
[ ] 5. Make sure that the BeadChips are properly seated in the BeadArray
Reader tray.
[ ] 6. Click Scan.
Scanning Process
BeadScan begins the BeadArray Reader Tilt and Align processes.
Once the Tilt and Align processes are complete, the Scan process
begins. Hover over any of the green dots in the closeup image to see the
relative intensity and the XY position. The red value should be at or close
to zero, because this is a one-color assay.
As the BeadArray Reader scans, the front panel blue Scanning indicator lights
flash in sequence.
Image
BeadChip on
BeadArray
Reader
The Illumina BeadArray Reader uses a laser to excite the fluor of the
hybridized single-stranded product on the b eads of the BeadChip
sections. Light emissions from these fluors are then recorded in high-
resolution images of the BeadChip sections. Data from these images
are analyzed using Illumina’s GenomeStudio Genotyping Module.
(26 pages)
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