PROCEED AMP3 User Manual download pdf (Page 19)

Infinium

HD Assay Gemini, Manual Protocol (Post-AMP)

Experienced User Card

Catalog # WG-901-3002 Page 19 of 28

Part # 11311015 Rev. A

Assemble Flow-Through Chambers

[ ] 1. If you have not done so yet, fill the BeadChip Alignment Fixture with 150

ml PB1.

[ ] 2. For each BeadChip to be processed, place a black frame into the Multi-

Sample BeadChip Alignment Fixture.

[ ] 3. Place each BeadChip to be processed into a black frame, aligning its

barcode with the ridges stamped onto the Alignment Fixture. Each

BeadChip should be fully immersed in PB1.

[ ] 4. Place a clear spacer onto the top of each BeadChip. Use the Alignment

Fixture grooves to guide the spacers into proper position.

[ ] 5. Place the Alignment Bar onto the Alignment Fixture.

[ ] 6. Use a laboratory air gun to quickly remove any accumulated dust from

the glass back plates just before placing them onto the BeadChips.

[ ] 7. Place a clean glass back plate on top of the clear spacer covering each

BeadChip. The plate reservoir should be at the barcode end of the

BeadChip, facing inward to create a reservoir against the BeadChip

surface.

[ ] 8. Attach the metal clamps to the Flow-Through Chamb ers as follows:

[ ] a. Gently push the glass back plate up against the Alignment Bar

with one finger.

[ ] b. Place the first metal clamp around the Flow-Through Chamber

so that the clamp is about 5 millimeters from the top edge.

[ ] c. Place the second metal clamp around the Flow-Through

Chamber at the barcode end, about 5 millimeters from the

reagent reservoir.

[ ] 9. Using scissors, trim the ends of the clear plastic spacers from the Flow-

Through Chamber assembly. Slip scissors up over the barcode to trim the

other end.

[ ] 10. Immediately wash the Hyb Chamber reservoirs with dH

O and scrub

them with a small cleaning brush, ensuring that no PB2 remains.

[ ] 11. Proceed to XStain HD BeadChip.

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PROCEED AMP3 User Manual Page 19