PROCEED AMP3 User Manual download pdf (Page 21)

Infinium

HD Assay Gemini, Manual Protocol (Post-AMP)

Experienced User Card

Catalog # WG-901-3002 Page 21 of 28

Part # 11311015 Rev. A

Estimated Time

Hands-on time: ~3 hours for 8–24 BeadChips

Dry time: 55 minutes

Consumables

XStain HD

BeadChip

Wash unhybridized and non-specifically hybridized DNA sample from

the BeadChips. Add labeled nucleotides to extend the primers

hybridized to the DNA. Stain the primers, disassemble the Flow-

Through Chambers, and coat the BeadChips for protection.

Item Quantity Storage Supplied By

RA1 10 ml for 1–8

BeadChips

20 ml for 9–16

BeadChips

30 ml for 17–24

BeadChips

-15 to -25°C Illumina

XC1 2 tubes

(per 8 BeadChips)

-15 to -25°C Illumina

XC2 2 tubes

(per 8 BeadChips)

-15 to -25°C Illumina

TEM 2 tubes

(per 8 BeadChips)

-15 to -25°C Illumina

XC3 50 ml for 1–8

BeadChips

100 ml for 9–16

BeadChips

150 ml for 24

BeadChips

Room

temperature

Illumina

STM (Make sure that all STM

tubes indicate the same stain

temperature on the label)

2 tubes

(per 8 BeadChips)

-15 to -25°C Illumina

ATM 2 tubes

(per 8 BeadChips)

-15 to -25°C Illumina

PB1 310 ml for 1–8

BeadChips

285 ml for 9– 24

BeadChips

Room

temperature

Illumina

XC4 310 ml for 1–8

BeadChips

285 ml for 9– 24

BeadChips

-15 to -25°C Illumina

Alconox Powder Detergent as needed Room

temperature

User

EtOH as needed Room

temperature

User

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Experienced User Card 1

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