PROCEED AMP3 User Manual download pdf (Page 17)

Infinium

HD Assay Gemini, Manual Protocol (Post-AMP)

Experienced User Card

Catalog # WG-901-3002 Page 17 of 28

Part # 11311015 Rev. A

Estimated Time

Hands-on time:

• 20 minutes per 8 BeadChips (16 samples)

• 30 minutes per 16 BeadChips (32 samples)

• 50 minutes per 24 BeadChips (48 samples)

Consumables

Preparation

[ ] Fill 2 wash dishes with PB1 (200 ml per wash dish). Label each dish

“PB1”.

[ ] Fill the BeadChip Alignment Fixture with 150 ml PB1.

[ ] Separate the clear plastic spacers from the white backs.

[ ] Clean the glass back plates according to the directions in the Infinium

Assay Lab Setup and Procedures Guide.

Steps

Wash BeadChip

[ ] 1. Remove each Hyb Chamber from the Illumina Hybridization Oven.

[ ] 2. Attach the wire handle to the rack and submerge the wash rack in the

first wash dish containing 200

ml PB1.

[ ] 3. Remove the Hyb Chamber inserts from the Hyb Chambers.

[ ] 4. Remove BeadChips from the Hyb Chamber inserts one at a time.

Wash BeadChip

Prepare the BeadChips for the staining process.

Item

Quantity

(per 8 BeadChips)

Storage Supplied By

PB1 550 ml Room

temperature

Illumina

Multi-Sample BeadChip

Alignment Fixture

1 Illumina

Te-Flow Flow-Through

Chambers (with black frames,

spacers, glass back plates,

and clamps)

1 per BeadChip Illumina

Wash Dish 8 BeadChips: 2 dishes

24 BeadChips: 6 dishes

Illumina

Wash Rack 8 BeadChips: 1 rack

24 BeadChips: 3 racks

Illumina

1 2 ... 12 13 14 15 16 17 18 19 20 21 22 ... 27 28

Experienced User Card 1

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PROCEED AMP3 User Manual Page 17

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