PROCEED AMP3 User Manual download pdf (Page 9)

Infinium

HD Assay Gemini, Manual Protocol (Post-AMP)

Experienced User Card

Catalog # WG-901-3002 Page 9 of 28

Part # 11311015 Rev. A

Estimated Time

Hands-on time: ~30 minutes per 48 samples

Incubation time: 2 hours

Consumables

Preparation

[ ] If frozen, thaw AMP3 plate to room temperature. Pulse centrifuge to

50 xg.

[ ] Thaw PA1 to room temperature. Centrifuge to 280 xg for 1 minute.

[ ] Preheat the heat block to 37°C, if it is not already.

[ ] Turn on the heat sealer.

[ ] Set the centrifuge to 4°C.

Steps

[ ] 1. Dispense 100 μl PA1 to each well containing sample.

[ ] 2. Seal the plate with the cap mat.

[ ] 3. Vortex the plate at 1600 rpm for 1 minute.

[ ] 4. Centrifuge to 50 xg at 22°C for 1 minute.

[ ] 5. Incubate at 37°C for 5 minutes.

[ ] 6. Add 300 μl 100% 2-propanol to each well containing sample.

[ ] 7. Seal the plate with a new, dry cap mat.

[ ] 8. Invert at least 10 times to mix contents.

[ ] 9. Incubate at 4°C for 30 minutes.

[ ] 10. Place the sealed AMP3 plate in the centrifuge opposite another plate of

equal weight.

[ ] 11. Centrifuge to 3000 xg at 4°C for 20 minutes.

Perform the next steps immediately, to avoid dislodging the blue pellet.

If any delay occurs, repeat the 20-minute centrifugation.

[ ] 12. Remove the cap mat.

[ ] 13. Decant the supernatant by quickly inverting the AMP3 plate and

smacking it down onto an absorbent pad.

[ ] 14. Tap the plate firmly on the pad several times over a period of 1 minute or

until all wells are completely devoid of liquid.

Precip AMP3

Precipitate the DNA sample using 2-propanol and PA1.

Item Quantity Storage Supplied By

PA1 1 tub e per

16 samples

2 to 8°C Illumina

100% 2-propanol 12 ml per 16 samples

40 ml per 48 samples

Room

temperature

User

1 2 3 4 5 6 7 8 9 10 11 12 13 14 ... 27 28

Experienced User Card 1

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