PROCEED AMP 5 User Manual download pdf (Page 19)

Single-Base Extension and Stain LCG BeadChip (Post-AMP)

In this process, you use RA1 reagent to wash away unhybridized and non-specifically

hybridized DNA sample. LX1 and LX2 are added to condition the BeadChip surface for the

extension reaction. Dispense EML reagent into the Flow-Through Chambers to extend the

primers hybridized to DNA on the BeadChip. This reaction incorporates labeled nucleotides

into the extended primers. 95% formamide/1 mM EDTA is added to remove the hybridized

DNA. After neutralization using the XC3 reagent, the labeled extended primers undergo a

multi-layer staining process on the Chamber Rack. Next, you disassemble the Flow-Through

Chambers and wash the BeadChips in the PB1 reagent, coat them with XC4, and then dry

them.

Estimated Time

Hands-on time: ~2 hours and 45 minutes for 8 BeadChips

Dry time: 1 hour

Illumina Infinium LCG Quad Assay, Manual Protocol

Experienced User Card

Part # 15025909 Rev. A

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PROCEED AMP 5 User Manual Page 19