PROCEED AMP 5 User Manual download pdf (Page 17)

Wash the BeadChip (Post-AMP)

[_] 1 If you have not done so, fill the Multi-sample BeadChip Alignment Fixture with 150 ml

PB1.

If more than four BeadChips will be processed, this 150 ml of PB1 can be reused for an

additional set of four BeadChips. You must use 150 ml of fresh PB1 for every additional

set of eight BeadChips.

[_] 2 For each BeadChip to be processed, place a black frame into the Multi-Sample BeadChip

Alignment Fixture pre-filled with PB1.

[_] 3 Place each BeadChip to be processed into a black frame, aligning its barcode with the

ridges stamped onto the Alignment Fixture.

NOTE

Inspect the surface of each BeadChip for residue left by the seal. Use a pipette tip

to remove any residue under buffer and be careful not to scratch the bead area.

[_] 4 Place a clear LCG spacer onto the top of each BeadChip. Use the Alignment Fixture

grooves to guide the spacers into proper position.

NOTE

Be sure to use the clear plastic spacers, not the white ones.

[_] 5 Place the Alignment Bar onto the Alignment Fixture.

The groove in the Alignment Bar should fit over the tab on the Alignment Fixture.

[_] 6 Place a clean LCG glass back plate on top of the clear spacer covering each BeadChip.

The plate reservoir should be at the barcode end of the BeadChip, facing inward to create

a reservoir against the BeadChip surface.

[_] 7 Attach the metal clamps to the Flow-Through Chambers as follows:

[_] a Gently push the glass back plate up against the Alignment Bar with one finger.

[_] b Place the first metal clamp around the Flow-Through Chamber so that the clamp is

approximately 5 mm from the top edge.

[_] c Place the second metal clamp around the Flow-Through Chamber at the barcode end,

approximately 5 mm from the reagent reservoir.

[_] 8 Using scissors, trim the ends of the clear plastic spacers from the Flow-Through Chamber

assembly. Slip scissors up over the barcode to trim the other end.

[_] 9 Immediately wash the Hyb Chamber reservoirs with DiH

O and scrub them with a small

cleaning brush, ensuring that no PB2 remains in the Hyb Chamber reservoir.

Illumina Infinium LCG Quad Assay, Manual Protocol

Experienced User Card

Part # 15025909 Rev. A

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PROCEED AMP 5 User Manual Page 17