PROCEED AMP 5 User Manual download pdf (Page 11)

Hybridize Multi BeadChip (Post-AMP)

Dispense the fragmented, resuspended DNA samples onto BeadChips. Incubate the

BeadChips in the Illumina Hybridization Oven to hybridize the samples onto the BeadChips.

Estimated Time

Hands-on time:

• 4x1 LCG BeadChip: ~40 minutes for 12 BeadChips (48 samples)

Incubation time: 16–24 hours

Consumables

Item Quantity Storage Supplied By

PB2 2 tubes (per 16

samples)

Room

temperature

Illumina

BeadChips 4 (per 16 samples) Illumina

Hyb Chambers 1 (per 16 samples) Illumina

Hyb Chamber gaskets 1 (per 16 samples) Illumina

Hyb Chamber inserts 4 (per 16 samples) Illumina

EtOH 330 ml User

Preparation

} Preheat the heat block to 95°C.

} Preheat the Illumina Hybridization Oven to 48°C.

Assemble the Hybridization Chambers

[_] 1 Place the resuspended AMP5 plate on the heat block to denature the samples at 95°C for

20minutes.

[_] 2 Remove the BeadChips from 2° to 8°C storage, leaving the BeadChips in their ziplock

bags and mylar packages until you are ready to begin hybridization.

[_] 3 During the 20-minute incubation, prepare the Hyb Chamber(s).

[_] a Place the BeadChip Hyb Chamber gaskets into the BeadChip Hyb Chambers.

[_] b Dispense 400 μl PB2 into the humidifying buffer reservoirs in the Hyb Chambers.

[_] c After you fill the Hyb Chamber reservoirs with PB2, place the lid on the Hyb

Chamber right away to prevent evaporation. The lid does not need to be locked

down.

[_] d Leave the closed Hyb Chambers on the bench at room temperature until the

BeadChips are loaded with DNA sample. Load BeadChips into the Hyb Chamber

within one hour.

Illumina Infinium LCG Quad Assay, Manual Protocol

Experienced User Card

Part # 15025909 Rev. A

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PROCEED AMP 5 User Manual Page 11